منابع مشابه
Cell-free Hemoglobin Synthesis
A cell-free protein-synthesizing system derived from rabbit reticulocytes is described which is dependent on the addition of transfer RNA for the translation of endogenous hemoglobin messenger RNA. Product analysis indicates that the system is active in the initiation of new chains. When hemoglobin is synthesized in the presence of a limiting amount of tRNA, there is a 50% decrease in (Y chain ...
متن کاملThe Synthesis of Hemoglobin in a Cell-free System.
28 J. C. Kendrew, G. Bodo, H. M. Dintzis, R. G. Parrish, H. Wykoff, and D. C. Phillips, Nature, 181, 660, 1958. 29 See J. Wyman, Jr., Advances in Protein Chem., 4, 410, 1948. 30 P. George and G. I. H. Hanania, Biochem. J., 52, 517, 1952. 31 P. George and G. I. H. Hanania, ibid., 55, 236, 1953. 32 H. Theorell, J. Am. Chem. Soc., 63, 1812, 1820, 1941. 33 See J. T. Edsall and J. Wyman, Jr., Biophy...
متن کاملSynthesis of hemoglobin in a cell-free system. I. Properties of the complete system.
The incorporation of Ci4-amino acids into protein, with cellfree systems containing ribosomes and soluble enzymes, has been studied extensively since the first reports by Zamecnik and Keller (1). This topic has been reviewed in detail by Hoagland (2). One limitation with many of the cell-free systems has been the failure to find incorporation into known proteins. Incorporation of CY-amino acids...
متن کاملCell-free hemoglobin synthesis. II. Characteristics of the transfer ribonucleic acid-dependent assay system.
A cell-free protein-synthesizing system derived from rabbit reticulocytes is described which is dependent on the addition of transfer RNA for the translation of endogenous hemoglobin messenger RNA. Product analysis indicates that the system is active in the initiation of new chains. When hemoglobin is synthesized in the presence of a limiting amount of tRNA, there is a 50% decrease in (Y chain ...
متن کاملCell-free hemoglobin: a novel mediator of acute lung injury.
Patients with the acute respiratory distress syndrome (ARDS) have elevated levels of cell-free hemoglobin (CFH) in the air space, but the contribution of CFH to the pathogenesis of acute lung injury is unknown. In the present study, we demonstrate that levels of CFH in the air space correlate with measures of alveolar-capillary barrier dysfunction in humans with ARDS (r = 0.89, P < 0.001) and i...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1970
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)63157-6